Ten Tips for Improving Western Blot Detection of Immunoprecipitation Samples

Immunoprecipitation (IP) experiments and the detection of IP samples by western blotting can be quite challenging at times. The ten tips covered in the following article will help you with the western blot detection of your IP samples and your overall experimental design. They include suggestions on how to select an appropriate antibody, what controls to use, how to choose the lysis buffer, how to denature your sample efficiently, among other things.

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Provides an overview of the IP process and hands-on-advice on how to design and control IP experiments