RNA-Seq

RNA sequencing (RNA-Seq) is a fundamental technique for transcriptome profiling, facilitating the detection of gene expression changes across sample groups. Traditional RNA-Seq of complex tissues, however, is not capable of deciphering the unique biological processes that take place within each cell. As such, single-cell RNA-Seq (scRNA-Seq) has emerged for the analysis of RNA molecules from individual cells, uncovering detailed insights that are unobtainable through bulk RNA-Seq.

Join Terence, a motivated undergrad, as he experiences the ups and downs of research using next-generation sequencing, learning valuable tips for successful NGS experiments along the way

RNA sequencing (RNA-Seq) is a fundamental discovery technique for determining the expression patterns of encoded genes. RNA-Seq has led to innumerable breakthroughs across disciplines; however, a need remains for new sequencing and library preparation technologies to address gaps in current methods. Read how improved access to RNA-Seq would benefit translational research and how a novel RNA library preparation chemistry can synergize with new sequencing platforms.

When it comes to tumor profiling, the poor quality and degradation of RNA in formalin-fixed paraffin-embedded (FFPE) samples are major limitations. To overcome this challenge, we tested a novel RNA-Seq workflow using the SEQuoia Complete Library Prep Kit and the SEQuoia Ribodepletion Kit. This workflow offers more complex transcriptome profiling, including both long and short RNA biotypes, to better represent the complete transcriptome.

RNA-Seq library preparation is a manually intensive, time-consuming process prone to bias from the multiple enzymes required. So, how can you trust your sequencing results? Bio-Rad is revolutionizing RNA-Seq library prep with the SEQuoia Express Stranded RNA Library Prep Kit – a three-hour, three-tube solution eliminating the need for multiple enzymes and reducing both the number of pipetting steps and protocol time. The key is an engineered, proprietary retrotransposon called SEQzyme. With this serial template jumping, or continuous synthesis, reaction you can obtain an unbiased, stranded RNA-Seq library constructed to accurately represent both messenger and non-coding RNA from your sample.

The SEQuoia RiboDepletion Kit employs novel technology to deplete ribosomal RNA while preserving rare transcripts and small RNAs, as well as minimizing the risk of degrading or low abundant samples. The SEQuoia RiboDepletion Kit also offers a streamlined workflow and multiplex compatibility, leading to significant time and cost savings compared to traditional methods. Watch this video to learn more.

Bio-Rad is excited to announce the launch of SEQuoia RiboDepletion Kit, the newest product in the SEQuoia portfolio of RNA-Seq sample preparation products. This is a stand-alone, post–library depletion kit that eliminates RNA-Seq library fragments that originated from ribosomal RNA sequences. The kit exhibits superior performance in retaining small RNAs while minimizing the loss of rare transcripts and RNA from limited samples, providing a better approach to whole transcriptome profiling. Furthermore, the cost of rRNA depletion can be significantly reduced by pooling and depleting multiple libraries in a single ribodepletion reaction.

mRNA-FISH, single-cell RT-qPCR, and single-cell RNA sequencing (RNA-Seq) have emerged as popular single-cell transcriptomics technologies. See how the Illumina® Bio-Rad® Single-Cell Sequencing Solution overcomes many of the challenges of currently available platforms by allowing high-throughput and scalable sequencing of even the most challenging sample types, including large cells and small species such as nuclei.