protocols and tips

Multiplexing an assay comes with its own complications in addition to the benefits it affords. Designing multicolor panels for flow cytometry analysis entails several considerations for a successful outcome. Here is a list of tips that can help you succeed in setting up and running a multicolor flow cytometry panel.

Harvesting of tissue culture cells could be challenging. Here is an easy protocol for efficient harvesting of cells for flow cytometry analysis.

When you have finished all your experiments and have the paper ready in your head, you need to consider several factors before sending it for publication. Here are some tips that Bio-Rad’s PrimePCR™ PCR Primers and Assays group put together for getting the paper published successfully.

Which antibody is the best to obtain a definitive and conclusive answer for my questions? How do I choose the best antibody for my purpose? These are standard questions that scientists ask time and time again. Here are a few tips to choose the most appropriate antibody for your specific application.

Immunoprecipitation (IP) is a commonly used protocol for concentrating and purifying proteins using specific antibodies and magnetic beads. Here is a simple IP protocol using Bio-Rad’s SureBeads Magnetic Beads.

A “device time-out” message on the Bio-Plex^® 200 System occurs when the instrument’s needle or probe cannot move correctly. Watch this video for several troubleshooting steps to try when this message occurs.

The high-throughput fluidics (HTF) alarm on the Bio-Plex^® 200 System indicates a problem with the instrument’s sheath fluid delivery system. See the actions needed to quickly resolve potential issues with this system and get back to your research!

During calibration of your Bio-Plex^® 200 System, you may receive a “failed to pressurize message.” In this short video, get some easy fixes for the most common issues prompting this message.

Usually when stripes appear on a gel image produced with a ChemiDoc XRS+ or ChemiDoc MP Imaging System, a misaligned filter is the cause. Learn the simple steps to correct this issue with this video from Bench Partners.

Amine coupling is the most commonly adopted technique to bind proteins to biosensor chips. Because of the possibility of the amine coupling reagents reacting with each other, these have to be premixed just before the injection and injected independently. Here are some tips to automatically coinject these reagents.