electrophoresis/western blotting

Evaluating potential protein therapies for purity and/or identity represents a vital and highly regulated step in biological drug development. Bio-Rad’s new Biologics Analysis Workflow™ offers a high-throughput, sensitive, and reproducible solution that is cGMP-compliant.

Research led by Prof. Ryan Jensen at Yale Medical School focuses on elucidating the links between BRCA gene mutations and breast cancer. Learn how researchers in his lab use Bio-Rad’s V3 Western Workflow to study interactions between BRCA proteins and the DNA repair protein RAD51. Understanding how these proteins interact in carcinogenesis and tumor growth will provide a new understanding of breast cancer mechanisms that could lead to new therapies.

Twenty years after the discovery of the link between BRCA gene mutations and breast cancer, researcher Ryan Jensen of the Yale Medical School is looking into the interactions between the DNA repair protein RAD51 and BRCA proteins using Bio-Rad’s V3 Western Workflow.

Removing host cell protein (HCP) contaminants represents an essential step in the production of biologics (therapeutic protein drugs), but has long been a time- and labor-intensive process. Here, we demonstrate a simpler, quicker, and more standardized workflow for evaluating anti-HCP antibodies using 2-D electrophoresis and western blotting.

2-D electrophoresis followed by immunodetection of protein spots using western blotting is adopted commonly in proteomics studies. Here we present step-by-step instructions for obtaining accurate overlaps between the gel and the blot using the PDQuest 2-D gel analysis software.

Bio-Rad’s 2-D electrophoresis workflow offers better results in a shorter time. Take a detailed look at this workflow by downloading the updated edition of our How-To Guide.

Bio-Rad’s innovative Gel Doc EZ imaging system features something unique that no other imaging system has – brains.

Learn how scientists in the Gene Regulation Laboratory at the Korea Advanced Institute of Science and Technology (KAIST) use the ChemiDoc MP imaging system in their work investigating the molecular machinery involved in epigenetic gene regulation.

When Dr. Fred Bauzon started his research, he worked with bioinformatics specialists to functionalize suitable gene candidates based on their expression levels with HMGCR transcript levels. All his experiments demonstrated a reduction in low-density lipoprotein receptor protein (LDLR) and candidate gene protein levels after the candidate gene was silenced. Here Bauzon discusses how TGX Stain-free precast gels have aided his research toward future drug targets for the therapeutic control of LDL uptake.

Learn how scientists in the Department of Animal Physiology at the University of Erlangen in Erlangen, Germany use the ChemiDoc MP imaging system in their work investigating sensory systems, in particular, the eye.