droplet digital PCR (ddPCR)

In this webinar, Dr. David Dodd describes how he and Prof. David Corey and colleagues at UT Southwestern Medical Center have used Droplet Digital™ PCR (ddPCR™) in their research into low-abundance targets with therapeutic potential for cancer and Huntington’s disease.

The QX100 Droplet Digital PCR system gives researchers an improvement in PCR precision and sensitivity. See how this innovative digital PCR system is enabling companies to create better products, improve workflow efficiencies, and validate results with greater accuracy than previous methods allowed.

Researchers at Sangamo BioSciences, Inc. explain the benefits of using third generation PCR as it applies to their research in the field of personalized medicine. Watch to see the latest generation of quantitative PCR in action.

Sangamo BioSciences, Inc., located in Richmond, California, is developing HIV therapeutics toward a functional cure using their proprietary zinc finger DNA-binding protein technology. Droplet digital PCR (ddPCR™) — or third generation PCR — offers a superior solution as it can detect target sequence within a single molecule, making it a powerful technology for low copy event detection.

Watch the step-by-step workflow for absolute quantification of DNA molecules using the third generation droplet digital PCR technology.

Droplet digital PCR is considered the third generation of PCR technology. This technology enables the quantification of absolute measure of target DNA molecules, rather than the relative measure obtained from real-time PCR. Absolute quantification is advantageous in several applications, such as measuring copy number variation, detecting rare sequences and mutations, and analyzing gene expression.

The one-step RT-ddPCR kit for probes creates a new paradigm for the precise quantitation of RNA by combining reverse transcription with Droplet Digital™ PCR (ddPCR™), providing an absolute measure of target RNA molecules with unrivaled precision and sensitivity

The McCarroll laboratory studies the biological effects of human genome polymorphism, seeking to define how genome variation influences gene expression and risk of disease.

Bio-Rad’s QX100™ Droplet Digital™ PCR system provides an absolute measure of target DNA molecules with unrivaled precision and accuracy.