chromatography

Multicolumn purifications are tedious and the manual operations during this process introduce variabilities, affecting the success of purification and reproducibility of results. This whiteboard animation explains how an automated multi-dimensional chromatography method can make this an easier process with very high success rates.

Dr. Mark Snyder, a leading expert in the field of protein purification, presents the purification process development for two very different targets, adenovirus and bacterial surface proteins. In both cases, a two-step column process results in a preparation with high yield and activity and very low host cell protein and DNA contamination. Conditions used make the methods suitable for large-scale production. In this webinar you will learn about: Advantages of mixed-mode resins Optimizing purification process development Procedures for purifying adenovirus and bacterial surface proteins  

Dr. John Bruning (University of Adelaide) and Dr. Jiansheng Wu (Genentech), two leading experts in the field of protein purification, share exciting examples of their research and recent chromatography tools they have used to demonstrate robust and improved efficiencies to their discovery and characterization protein purification workflows.

The separation of monomers from product-related impurities is quite challenging in process separations. It requires a thorough screening and an efficient resin to get efficiency in the purification process. Review the performance data of Bio-Rad’s mixed mode CHT™ Ceramic Hydroxapatite in achieving process separation efficiency and how it compares with various Capto media.

The best chromatography media should yield high protein purity and product recovery as well as be versatile enough to purify difficult samples. Bio-Rad’s CHT™ Ceramic Hydroxyapatite achieves this by virtue of being able to interact with the proteins through multiple modes, making the process extremely efficient. The whiteboard animation describes the mode of action of CHT.

What can chromatography peaks tell us? From the quality of column packing to the purity of separated proteins, a lot can be gleaned from the peaks. Here are some definitions that can help decipher what chromatography peaks can tell us.

Here are some ways you can detect DNA contamination during the protein purification process.

Explore some of the key considerations for chromatography method development.

Which valves, pumps or detectors are optimal for your FPLC system? How do you achieve optimal gradient performance? Here are some tips.

Philip Chapman of Bio-Rad Laboratories discusses the advantages of automated multidimensional (Multi-D) chromatography compared to traditional multistep purification strategies.