chromatography

A day in the life of Maria, a post-graduate scientist. Both Maria and her PI are annoyed with their unreliable, out-dated protein purification system, and her PI asks Maria to buy them a new one. She struggles to find a system that meets all their needs until she remembers that Bio-Rad has a new next-generation chromatography system that will work for everyone!

Is spending hours in the coldroom just an inevitable part of doing chromatography? The engineers and designers at Bio-Rad believed that, with a little innovation, the answer could be “no.” Part two of The Chromatography Chronicles reveals how they freed the NGC™ medium-pressure chromatography system — and its users — from the coldroom’s chilly confines.

Watch the protein purification election night special parody! Find out which chromatography system wins. Laugh Out Loud at the terrible protein purification “pundit” and Vote Yes for Better Chromatography “Fanatics.” Learn what values and system features were appreciated most by voters.

Whether through unreliability, lack of workflows, or poor design, chromatography systems can cause major hassles for any lab involved in purifying proteins. Would Bio-Rad be able to solve these longstanding problems? This new series takes an inside look at the development of the NGC medium-pressure chromatography system. This month: bringing customers into the design process.

The Fraser lab at University of California Davis tested Bio-Rad’s NGC system for the purification of ribosomal complex proteins in their studies of protein translation mechanisms. The NGC system enabled these researchers to significantly increase their protein purification throughput and save valuable time in the lab.

The Fraser Laboratory in the Dept. of Molecular and Cellular Biology at UC Davis studies protein translation mechanisms by purifying diverse ribosomal proteins and reconstructing the translation complex in vitro. The lab utilized Bio-Rad’s NGC system to overcome specific purification difficulties with their protein while beta-testing the equipment. The NGC system enabled the group to increase their throughput by 25% and also saved substantial training time.

This year the California Separation Science Society will present its annual Scientific Achievement Award to Professor Stellan Hjertén of Uppsala University. The award dinner will be held November 3, 2011 at the Waterfront Hotel in Jack London Square.

The C-96 autosampler improves the efficiency of protein purification workflows by enabling automated sample application. The straightforward tray designs are easy to use; the standard tray accommodates 84 sample vials (5 ml each) plus three additional vials (10 ml each) for mixing and dilution protocols.

Nuvia S media is a high-capacity strong cation exchanger (CEX) developed to operate over a range of experimental conditions, allowing scientists to scale up from laboratory- to process-scale more quickly and easily. Our data demonstrate that the high capacity and superb purification of Nuvia S media can significantly improve productivity by offering the robustness, capacity, and speed for processing large load volumes with smaller columns and less buffer consumption.