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alt Melanoma is an aggressive cancer where early detection is essential for patient survival. The Facchiano lab is developing new anti-proliferation molecules against melanoma and is working to identify new molecular markers at the very early stages of pathology. Here Dr Antonio Facchiano discusses the merits of using the Bio-Plex technology to measure dose-dependent changes in expression levels of growth and angiogenic factors in cell lysates and supernatants.

The latest version of the CFX Manager software provides a variety of usability enhancements and powerful new data analyses so users can get the most out of each run.

Learning to use analysis software can be time-consuming and following a manual can be challenging.

Mini-PROTEAN® TGX Stain-Free™ precast gels are now available in gradient format. This unique gel formation allows for fast gel electrophoresis and blotting, and does not require staining and destaining for imaging.

PrimePCR™ assays set a new standard for real-time PCR. Bio-Rad® collaborated with the leaders in real-time PCR to design, optimize, and experimentally validate every primer pair.

Sangamo BioSciences, Inc., located in Richmond, California, is developing HIV therapeutics toward a functional cure using their proprietary zinc finger DNA-binding protein technology. Droplet digital PCR (ddPCR™) — or third generation PCR — offers a superior solution as it can detect target sequence within a single molecule, making it a powerful technology for low copy event detection.

The updated Image Lab image acquisition and analysis software provides improved tools for total protein normalization using the stain-free technology.

Detecting dilute protein samples using standard protein gel stains can be problematic. One method of enabling increased sample capacity on gels is to increase the well size. The newly introduced 4x Laemmli sample buffer improves sample loading capacity, improving the detection of low-abundance proteins.

Analyzing antibody binding to histidine-tagged proteins is not always easy because of inherent problems such as nonspecific binding and loss of analyte response associated with these tagged proteins. Optimization of these factors is key to precisely measuring the binding kinetics of these proteins.

Many instruments offer multiplexing capability for biomarker measurements. But to handle and make sense of the huge amount of multiplex data requires sophisticated software like Bio-Plex Data Pro, which simplifies organization, import, sorting and visualization of data, and performing calculations and statistical analyses.