Multiplex Analysis of Cell Death Markers Using Bio-Plex Pro™ RBM Apoptosis Assays
Bio-Rad’s new Bio-Plex Pro RBM Apoptosis Multiplex Assays enable complex, quantitative analyses of biomarkers in the intrinsic apoptosis pathway. Learn more about these biomarkers and view sample data and performance characteristics of these assays in this detailed technical report.
Genomic Variation Analysis with Droplet Digital PCR: Success Stories
Researchers are having success applying Droplet Digital PCR (ddPCR™) technology across many different fields. Here are two such success stories, one involving the application of ddPCR in detecting mutations in iPS cells, the other in predicting allele-specific variation in human brain development.
Brighter Precision Plus Protein™ Dual Color Standard — The Brightest Protein Marker Available
Take a quick look at Bio-Rad’s brighter Precision Plus Protein™ Dual Color Standard with this article comparing this standard to other available protein markers. Plus, find out why the ability to visualize a protein standard after reprobing matters to one researcher!
Better, Faster, and Less Expensive Gene Expression Analysis Using Multiplex One-Step RT-qPCR: A Case Study of Colorectal Cancer
Conducting a triplex one-step RT-qPCR assay promises a more efficient and economical analysis of gene expression compared to running three individual assays. This technical report describes a colorectal cancer case study where a triplex multiplex assay was validated.
Ligand Immobilization in Protein Interaction Studies — An Unattended Amine Coupling Protocol with Automatic Coinjection Activation
Amine coupling is the most commonly adopted technique to bind proteins to biosensor chips. Because of the possibility of the amine coupling reagents reacting with each other, these have to be premixed just before the injection and injected independently. Here are some tips to automatically coinject these reagents.
Bio-Rad’s Universal Real-Time PCR App – Everything You Need to Know about Real-Time PCR at Your Fingertips
Bio-Rad’s Universal Real-Time PCR Kits and Reagents work on all real-time PCR systems. Now you can easily compare performance data and get supporting documents for these kits and reagents using Bio-Rad’s new tablet and web app. Learn more and download the app here.
Developing Bio-Rad’s S3™ Cell Sorter: The Great Cell Sorting Redesign
Cell sorting technology is not new. However, sometimes taking a new look at a mature technology brings unexpected results. See how we reimagined drop delay calculation, rethought software and automation, and more during the design and development of Bio-Rad’s S3 Cell Sorter.
Protein Purification Workflow Development Using Bio-Rad’s NGC™ Chromatography System
While using affinity tagging is the preferred method to achieve highly pure protein through recombinant expression, this approach isn’t feasible for all proteins. This tech report describes workflow optimizations using the NGC Chromatography System that allow users to achieve high purities with untagged proteins.
Unlocking the Structures of Natural Killer Cell Receptors with the NGC™ Chromatography System
Structural biologist Petr Novák employs the latest techniques to model natural killer cell receptor molecules, research that may one day result in powerful therapies to treat cancer and infections. Go inside the fascinating world of structural characterization and find out how Novák has integrated the NGC™ chromatography system into his effort to visualize these molecules in vivid detail.
Novel Liposome-Capture Surface Chemistries to Analyze Drug-Lipid Interaction Using the ProteOn™ XPR36 System
Surface plasmon resonance (SPR) biosensors offer a label-free technique for profiling biomolecular interactions, including those between drugs and liposomes. Here we describe the novel surface chemistries that make capturing liposomes possible, and explore the application of the liposome capturing kit in analyzing liposome-small molecule interactions.