Real-Time qPCR/PCR Video

Discover the simplicity of crafting your own custom PrimePCR plate with Bio-Rad. Follow along with our intuitive plate wizard, which helps you select your plate type (96- or 384-well) and compatible qPCR instrument for precise assay delivery. Watch this video to see how.

The PTC Tempo Thermal Cycler represents the next generation of conventional PCR thermal cyclers from Bio-Rad. Engineered to maintain thermal performance, the PTC Tempo Thermal Cycler offers the flexibility to scale with your laboratory. The design features a refreshed, intuitive user interface and flexible connectivity options for streamlining protocol management. A motorized lid enables automation, taking your PCR to the next level with modern instrument control.

Through the CFX Duet Real-Time PCR System, our newest generation of quantitative PCR (qPCR) instrumentation, this technology is now even more accessible for molecular biologists and other professionals doing essential qPCR. With CFX Maestro Software, you can perform duplex detection, gene expression, and genotyping, all without paying for additional optics you don’t need. Check out our video tour of the new CFX Duet System.

From flow cytometry to chromatography to western blot imaging to PCR plastics, learn more about Bio-Rad and see what’s possible in your research.

Learn about the exciting new field of long noncoding RNA (lncRNA) and how this newly discovered class of RNA compares to messenger RNA (mRNA).

Traci Lyons describes how PrimePCR Assays ended her laboratory’s struggle to find a qPCR assay that could reliably detect transcription of the cyclooxygenase-2 (Cox-2) gene, which they had identified as potentially prometastatic. Using the PrimePCR Assay for Cox-2, researchers in her lab were finally able to verify cyclooxygenase-2 knockdown, which is a necessary step toward confirming its role in breast cancer metastasis.

What’s the optimal melting temperature for a hydrolysis probe? How long should your probe be? And why should you avoid 5′ guanines like the plague? This episode answers these questions and gives you five simple tricks for designing the perfect hydrolysis probe.

Follow these six simple tips and you’ll increase the reproducibility of your qPCR experiments.

Are you using ethanol to prep your bench for qPCR? Watch this episode to find out why that might be a bad idea. We’re sharing seven tips for minimizing contamination of qPCR experiments.

How long should a qPCR primer be and what types of sequences should you avoid? In this episode we share eight tips and tricks for primer design for your qPCR experiments.