Lab Notes

What’s the optimal melting temperature for a hydrolysis probe? How long should your probe be? And why should you avoid 5′ guanines like the plague? This episode answers these questions and gives you five simple tricks for designing the perfect hydrolysis probe.

Follow these six simple tips and you’ll increase the reproducibility of your qPCR experiments.

Are you using ethanol to prep your bench for qPCR? Watch this episode to find out why that might be a bad idea. We’re sharing seven tips for minimizing contamination of qPCR experiments.

How long should a qPCR primer be and what types of sequences should you avoid? In this episode we share eight tips and tricks for primer design for your qPCR experiments.

From making sure that your lysis buffer is compatible with your protein quantitation method to advice on when you do and don’t have to worry about proteases, we share helpful tips for developing a sample preparation protocol.

Sonicator or freeze-thaw? Detergent or enzymatic lysis? Find out how to pick the right cell disruption method for your experiment.

Watch this episode of LabNotes for helpful tips like how to pour the stacking and resolving gel at the same time.

We share five simple tricks that address common gel running woes, from samples that float out of wells to sample buffers that turn yellow.

Unsure about what percentage SDS-PAGE gel to pick for your experiment? We share four tips that make picking the perfect gel a piece of cake.

In this episode, we share tips and tricks for minimizing protein degradation during cell lysis and sample preparation.