Technical Reports

This tech report shows how qPCR can lead to erroneous conclusions regarding differences in MCM7 gene expression between normal and tumor human breast cancer samples if the key steps set out in the MIQE guidelines are not followed.

This tech report compares in-gel and off-gel separation of proteins in terms of overall speed and simplicity of the workflow, resolution of the separation, and depth of proteome coverage provided. Results found that as well as higher peptide yields, in-gel separations were better suited to take full advantage of downstream analysis methods.

This tech report demonstrates the advantages of independently controlled voltage and current parameters for each focusing tray in optimizing IEF conditions for a complex, insoluble membrane protein fraction.

This report demonstrates that the TC10 automated cell counter is a suitable alternative to the Coulter Counter and hemocytometer for the preparation of custom Bio-Plex assays.

This tech report describes an automated method of RNA extraction using the Aurum total RNA 96 kit and its use in a magnetic–bead based automated process. Results show that throughput can be easily increased with the Aurum total RNA 96 kit and that the automation protocols yield more reliable results, particularly when high sample volumes are used.

Not all cDNA synthesis kits have the same capacity to protect the integrity of RNA. An optimal level of RNase inhibitor is critical for powerful RNase inhibition and efficient reverse transcription. The iScript™ cDNA synthesis kit comes with a 5x iScript reaction mix and iScript reverse transcriptase, which is also blended with a potent RNase inhibitor.

This tech report presents the results of a comparative study between the TC10 automated cell counter and the classical LDH assay to measure cell viability in suspension cells. Results show that the TC10 cell counter can replace the LDH assay to measure both the viability of cell suspensions and the cytotoxic effects of various compounds.