Real-time qPCR/PCR

Bio-Rad is proud to introduce our new RT-PCR testing kits for COVID-19. These all-in-one kits are designed for sensitive, specific detection of SARS-CoV-2 alone, or simultaneous detection of SARS-CoV-2, influenza A, and influenza B. Read on to learn about each kit’s features.

The Reliance Select cDNA Synthesis Kit is the newest reverse transcription product from Bio-Rad. This high-performance kit features Reliance Reverse Transcriptase, a novel chimeric enzyme that delivers superior results across a broad dynamic range, even with challenging samples. The Reliance Select cDNA Synthesis Kit delivers more accurate reverse transcription quantitative PCR (RT-qPCR) results, even from samples containing PCR inhibitors or degraded RNA.

Coming soon: The next iteration in Bio-Rad’s tradition of qPCR excellence. The compact CFX Opus Real-Time PCR System is more connected, consistent, and easier to use than ever before, and is available in 96- and 384-well formats. The CFX Opus Systems launch with our new BR.io cloud platform and the latest version of CFX Maestro Software, for complete instrument control and data analysis capabilities.

Reference gene selection and validation are very important for gene expression studies that involve different samples or experimental conditions and therefore are absolutely necessary before any further exploration. See how PrimePCR Reference Gene Selection Panels and CFX Maestro Software provide an easy-to-use system with which to select appropriate reference genes.

Protein thermal shift assays enable quick and easy buffer optimization for increased protein stability. See how Bio-Rad’s family of CFX Real-Time PCR Detection Systems can measure protein thermal stability with higher throughput and more buffer systems than traditional circular dichroism detection.

One-step multiplex RT-qPCR is a technique used to rapidly quantify multiple targets directly from RNA in a single reaction. But proper optimization and validation is essential for its success. These five tips will have you designing primers and probes, selecting reporters, and validating and optimizing your experiments like a pro.

RT-qPCR is a well-established method for the detection, quantification, and typing of different microbial agents. However, when dealing with the challenges of pathogen detection, not all RT-qPCR reagents are created equal. See how we used a novel one-step multiplex RT-qPCR supermix to demonstrate sensitive codetection of viral RNA and DNA targets in a multiplex setting.

qPCR has become ubiquitous for nucleic acid detection and quantification and remains the gold standard for a wide array of applications. To help you publish high-quality, reproducible data that reflect true experimental conditions, we’ve developed this comprehensive guide to performing the ultimate qPCR experiment.

Using RT-qPCR for RNA, though critical in several industries, is often met with challenges. Many current options don’t allow for multiplexing or high-throughput operation. Bio-Rad’s new Reliance One-Step Multiplex Supermix was designed to grant researchers the expanded, efficient capabilities they need in RT-qPCR. This article presents some of the performance data from the new supermix and describes how exceptional it is at multiplex detection.

Passive reference dyes are included in qPCR/RT-qPCR supermixes to compensate for pipetting errors and thermal and/or optical differences in real-time instruments. Our universal line of reagents eliminates the need for spiking in dyes or having to worry about how much ROX your instrument needs. See how your qPCR experiments can also generate more reliable data and be reproducible by anyone.