Protocols and Tips

Ligand binding assays are utilized throughout the large-molecule drug development process. However, their robustness, accuracy, and reproducibility depend on the quality of critical reagents. See how you can ensure their consistent characterization, particularly during early drug development stages.

Follow the five tips in this article before embarking on your multiplex immunoassay journey to generate accurate, efficient, and biologically relevant results. From help designing experiments to acquiring accurate data, this article has it all.

Digital PCR offers a highly sensitive alternative to conventional qPCR, enabling absolute quantification of nucleic acids and rare allele detection. The tips in this article help you set up and optimize your digital PCR experiments when using droplet partitioning technology.

Immunoprecipitation (IP) experiments and the detection of IP samples by western blotting can be quite challenging at times. The tips in this article help you set up and optimize your overall IP experimenta design.

A good and complete transfer of proteins from gels to membranes is crucial for the success of western blotting. Here are six useful tips for successful transfer of proteins.

A biomolecule protein purification workflow requires more than one resin. The trick is to find the combination of resins that provides the highest purity in the least number of steps and at the lowest cost. Here are some guides to select the optimal resins for the purification of monoclonal antibodies, native and recombinant proteins, Ig G and mammalian and adenovirus, and for removing aggregates and endotoxins.

It is imperative to analyze both the transcriptional and translational profiles for a complete understanding of the functioning of genes. Here we describe a simple protocol that enables parallel analysis of both RNA and protein from single cell culture lysates. See how this workflow could save time and money and yield more reliable data than the traditional protocols used.

Picking the right gel for your application is not easy. It depends on so many factors, such as the purpose of your application, the size of your protein, what kind of buffer you need to have in your sample etc. Here we provide a guide for you to choose the best Bio-Rad gel based on all these factors.

Multiplex immunoassay development requires a lot of validation and standardization steps. Several factors can influence the performance of these assays. Some factors that need to be considered are mentioned here.

How can we easily identify biomarkers for different immune cell types? This color-coded poster makes identification of mouse lineage biomarkers easy.