Articles

The ProteOn XPR36 protein interaction analysis system was introduced in 2006 to provide a novel array technology to researchers conducting label-free analysis of proteins. The ProteOn system is a high-throughput surface plasmon resonance-based screening tool for a wide range of applications from small-molecule drug discovery to antibody kinetic ranking, epitope binning, and epitope mapping.

The ChemiDoc MP imaging system enables stain-free operation and lets you visualize proteins at every stage of your experiment. Its flexibility and sensitivity are complemented by a simple, intuitive operation that integrates seamlessly into your workflow.

Introducing Bio-Rad’s PROTEAN i12 IEF system. Each channel in the i12™ focusing tray is powered by its own power supply, enabling precise control over each IPG strip. This makes it possible to run different sample types, different gradients, and multiple protocols all at the same time.

This tech report demonstrates the advantages of independently controlled voltage and current parameters for each focusing tray in optimizing IEF conditions for a complex, insoluble membrane protein fraction.

Immun-Blot LF (low fluorescence) PVDF membranes are protein blotting membranes optimized for fluorescent and multiplex fluorescent applications. They offer high signal-to-background ratio, low autofluorescence, and superior protein retention to maximize blot detection sensitivity and enable downstream quantitation.

This report demonstrates that the TC10 automated cell counter is a suitable alternative to the Coulter Counter and hemocytometer for the preparation of custom Bio-Plex assays.

Identifying Cardiac Defect–Related Protein SignaturesAugust 15, 2011 “The first opportunity I had to lay my hand on a human heart I knew that was that — I knew that I wanted to know how the heart works, but more importantly, how it doesn’t work,” says Dr Francis Spinale, describing his inspiration to both research and treat cardiac disease. Using the Bio-Plex® suspension array system, researchers in his laboratory are discovering protein signatures that have the potential to inform decision strategies in cardiac patient treatment.

Dr. Steven Kornblau has been on faculty at MD Anderson Cancer Center in Houston, Texas, for 20 years, where he divides his time between research into further understanding leukemia disease states, and a clinical practice where he treats a small number of patients with the disease. Recently, his laboratory used the Bio-Plex® suspension array system to discover how cytokine and chemokine profiles alone can be prognostic for patients with the disease, and therefore have to potential to assist development of personalized treatments.

This tech report describes an automated method of RNA extraction using the Aurum total RNA 96 kit and its use in a magnetic–bead based automated process. Results show that throughput can be easily increased with the Aurum total RNA 96 kit and that the automation protocols yield more reliable results, particularly when high sample volumes are used.

The C-96 autosampler improves the efficiency of protein purification workflows by enabling automated sample application. The straightforward tray designs are easy to use; the standard tray accommodates 84 sample vials (5 ml each) plus three additional vials (10 ml each) for mixing and dilution protocols.