Articles

Academic labs don’t have the money to do full-scale validations of antibodies. What tricks can small biotechs teach them about validation? Find out.

Gene expression analyses become complicated and extremely time consuming when large numbers of genes need to be analyzed. Here we present a semi-automated qPCR workflow with reduced setup and processing times using Bio-Rad’s PrimePCR™ Panels and CFX Automation System II Plate Handler.

Harvesting of tissue culture cells could be challenging. Here is an easy protocol for efficient harvesting of cells for flow cytometry analysis.

From using nonspecific polyclonal antibodies to developing monoclonal antibodies from immortalized antibody-producing cells to antibody engineering and generation of antibodies using an animal-free library with billions of antibody fragments to choose from, the antibody technology has seen tremendous advances. Explore where this journey started and what the current trends are and what the human combinatorial antibody library (HuCAL) technology destination has to offer!

Some of the frustrations in protein purification stem from unmet chromatography needs, such as automation, productivity, throughput, and reproducibility among others. Bio-Rad’s new Multi-D chromatography method addresses these and provides solutions to faster, efficient and more reliable protein purification.

Genome editing using tools such as CRISPR/Cas9 and TALEN has gained enormous popularity in recent days due to their ability to create precise deletions or insertions. However when the frequency of these gene edits are really small, an ultrasensitive method like ddPCR™ is needed for accurate quantification. This technote explains how Droplet Digital™ PCR is used for the detection of HDR and NHEJ alleles generated using CRISPR/Cas9 and TALEN editing systems.

Few discoveries have changed the pace of discovery and the types of questions we can ask as drastically and quickly as CRISPR-based genome editing. But where did CRISPR come from? How was it adapted to become such a game-changing genome editing tool? And why are some of its greatest proponents alarmed?

Why western blotting, a technique used in every day research, is still not as straightforward as it seems? Well, it’s mainly because of the antibodies. They may not work in all samples, a lot of standardization needs to be done and there are several other unknowns. Bio-Rad, the leader in western blotting, has addressed these concerns in its newest line of antibodies, the PrecisionAb™ Antibodies. See the challenges and the Bio-Rad solution.

Finding ways to slow or reverse aging intrigues scientists and non-scientists alike. In recent studies, merging the circulatory systems of young and old mice rejuvenated skeletal muscle, brain, and cardiac cells, indicating the presence of a systemic “anti-aging factor” initially thought to be growth differentiation factor 11 (GDF11). However, a recent publication showed that GDF11 actually promotes aging, emphasizing the need for further integrative analysis of the aging process.

When you have finished all your experiments and have the paper ready in your head, you need to consider several factors before sending it for publication. Here are some tips that Bio-Rad’s PrimePCR™ PCR Primers and Assays group put together for getting the paper published successfully.