Cell Biology

Part III of our cell health series describes the three main autophagy mechanisms and reviews the various methods for assessing macroautophagy and autophagic flux in cells.

Monocytes play key roles in mediating innate and adaptive immune responses. Here is an overview of the types, functions, process of recruitment, and the role of monocytes in disease.

In this second part of the series on assessing the health of your cells, we review the different types and stages of apoptosis, and the various methods of assessing apoptosis of cells.

Macrophages are important players in the phagocytosis process in the immune system. In this detailed review, we provide an overview on the different types of macrophages, the signaling molecules involved in the polarization of M1 and M2 macrophage subsets, and more.

In this multi-part series on assessing the health of your cells, we review different factors to be considered in your assessments such as cell viability, proliferation, apoptosis, and autophagy. The first part covers the methods for assessing viability and proliferation and their advantages, and discusses some common considerations.

To run a core flow cytometry lab is no easy task. There is always a balance between running simple sorts and developing new assays for answering complicated questions that demand pushing the limits of existing assays and the limitations of the sorters. See how Andy Riddell, a flow core manager, pushes the limits of his existing assays and how Bio-Rad’s S3e™ cell sorter is allowing him to accomplish that.

It’s always good to get the basics straight. Here, we’ve defined some flow cytometry terms that might have eluded you.

Multiplexing an assay comes with its own complications in addition to the benefits it affords. Designing multicolor panels for flow cytometry analysis entails several considerations for a successful outcome. Here is a list of tips that can help you succeed in setting up and running a multicolor flow cytometry panel.

Harvesting of tissue culture cells could be challenging. Here is an easy protocol for efficient harvesting of cells for flow cytometry analysis.

Using traditional fluorescence microscopes for everyday cell imaging can be frustrating, with a lot of time spent in the darkroom mastering a highly specialized piece of equipment, all to accomplish relatively simple tasks. The ZOE Fluorescent Cell Imager eliminates the complexities of cell imaging by combining the ease of use of a personal tablet with the power of an inverted microscope. Check it out.