Bioradiations Staff
The new CFX96 Touch Deep Well real-time PCR detection system joins Bio-Rad’s real-time PCR detection system family as the system for large-volume qPCR reactions (up to 125 µl). The system can accurately discriminate up to five targets in a single reaction well by using Bio-Rad’s solid-state optical technology (Figure 1). The optical system scans just above the sample plate to illuminate and detect from each well with high sensitivity and no cross talk.
In addition to using industry-leading optical technology, the system offers excellent thermal performance and uniformity for large-volume reactions by using Bio-Rad’s patented reduced-mass sample block (Figure 2). This sample block heats and cools more quickly than standard blocks, so average ramp rates are increased and overall run times are reduced. The system also incorporates a built-in thermal gradient for easy assay optimization in a single run.
The CFX96 Touch Deep Well system includes the easy-to-use C1000 Touch™ chassis to create a truly stand-alone qPCR system. Runs can be quickly and easily set up using the intuitive LCD touch screen menus (Figure 3) and amplification traces can be viewed on the LCD screen in real time while a run is in progress (Figure 4) providing a truly stand-alone qPCR system.
Fig. 1. Linearity of five-target multiplex detection. A–E, fluorescence data from a series of tenfold dilutions of plasmid DNA (109–103 copies) amplified using reporter dyes to monitor five targets in a 75 µl reaction volume. /2012/10/color_rectangle_darkblue.jpg” style=”width: 7px; height: 7px; float:none; display: inline; margin-right: 0px; ” /> FAM/cyclophilin; /2012/10/color_rectangle_green.jpg” style=”width: 7px; height: 7px; float:none; display: inline; margin-right: 0px; ” /> HEX/GAPDH; /2012/10/color_rectangle_red.jpg” style=”width: 7px; height: 7px; float:none; display: inline; margin-right: 0px; ” /> Texas Red/actin; /2012/10/color_rectangle_purple.jpg” style=”width: 7px; height: 7px; float:none; display: inline; margin-right: 0px; ” /> Cy5/tubulin; /2012/10/color_rectangle_darkred.jpg” style=”width: 7px; height: 7px; float:none; display: inline; margin-right: 0px; ” /> Quasar 705/IL-1β; F, standard curves generated from data in A–E, reaction efficiencies range from 100 to 102%. Cq, quantification cycle; RFU, relative fluorescence units.
Fig. 2. Excellent Uniformity. IL-1β plasmid template was diluted to 105 copies/reaction and amplified in the presence of a FAM-labeled detection probe with IQ™ supermix. Graph shows 96 replicates of 100 µl reactions. Average Cq = 25.14 ± 0.10. RFU, relative fluorescence units.
Fig. 3. Setting up runs. Get started quickly with the intuitive navigation options.
Fig. 4. Real-time monitoring of data. Easily monitor traces in real time on the large touch screen.
Data analysis can be performed using the powerful CFX Manager™ software. The software offers easy run setup and advanced gene expression data grouping tools such as bar chart, clustergram, scatter plot, volcano plate, and heat map (Figures 5 and 6) for all Bio-Rad’s real-time PCR detection systems.
Fig. 5. Data analysis. Quickly start, repeat, or begin analyzing a run in three clicks or fewer from the new Startup Wizard in the CFX Manager software.
Fig. 6. Gene expression analysis using the CFX Manager software. Explore the gene expression analysis tools in the CFX Manager software to quickly discover patterns of regulation in your data and identify gene targets for further investigation (bar charts, clustergrams, scatter plots, volcano plots, and heat maps).
Specifications
The following chart displays the specifications of all available real-time PCR detection systems from Bio-Rad.
| MiniOpticon™ | CFX Connect | CFX96 Touch | CFX96 Touch Deep Well | CFX384 Touch | |
| Base thermal cycler | MJ Mini™ | CFX Connect | C1000 Touch™ | C1000 Touch | C1000 Touch |
| Sample volume | Up to 100 µl | Up to 50 µl | Up to 50 µl | Up to 125 µl | Up to 30 µl |
| Excitation range | 470–500 nm | 450–535 nm | 450–684 nm | 450–684 nm | 450–650 nm |
| Multiplex capability | Up to 2 targets | Up to 2 targets | Up to 5 targets | Up to 5 targets | Up to 4 targets |
| Gradient range | 35–99°C | 30–100°C | 30–100°C | 30–100°C | 30–100°C |
Ordering Information
| Catalog# | Description |
| 1854096 | CFX96 Touch Deep Well Real-Time PCR Detection System, includes C1000 Touch thermal cycler chassis, CFX96 Deep Well™ optical reaction module, CFX Manager software, license for qbasePLUS software, communication cable, reagents, consumables |
| 1854095 | CFX96 Touch Deep Well Real-Time PCR Detection System, includes C1000 Touch thermal cycler chassis, CFX96 Deep Well optical reaction module, CFX Manager software, license for qbasePLUS software, communication cable |
| 1844096 | CFX96 Deep Well Optical Reaction Module, for use with C1000 Touch thermal cycler chassis, includes CFX Manager software, license for qbasePLUS software, communication cable, reagents, consumables |
Notice regarding Bio-Rad thermal cyclers and real-time systems: Purchase of these instruments conveys a limited non-transferable immunity from suit for the purchaser’s own internal research and development and for use in human in vitro diagnostics and all other applied fields under U.S. Patent Number 5,475,610 (Claims 1, 44, 158, 160–163, and 167 only), or corresponding claims in its non-U.S. counterpart, owned by Applera Corporation. No right is conveyed expressly, by implication, or by estoppel under any other patent claim, such as claims to apparatus, reagents, kits, or methods such as 5′ nuclease methods. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Bio-Rad’s real-time thermal cyclers are licensed real-time thermal cyclers under Applera’s U.S. Patent Number 6,814,934 B1 for use in research, human in vitro diagnostics, and all other fields except veterinary diagnostics. Bio-Rad’s real-time thermal cyclers are covered by one or more of the following U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 6,767,512 and 7,074,367.
Cy is a trademark of GE Healthcare group companies. FAM is a trademark of Applera Corporation. Quasar is a trademark of Biosearch Technologies, Inc. SYBR is a trademark of Life Technologies Corporation. Bio-Rad Laboratories, Inc. is licensed by Life Technologies Corporation to sell reagents containing SYBR Green I for use in real-time PCR, for research purposes only. Texas Red is a trademark of Invitrogen Corporation.
Purchase of iQ supermix includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser’s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.