Maximizing Metastasis Insights with a Novel Integrated Workflow for CTC Enrichment and Enumeration

Characterizing metastasis remains key for understanding and eradicating cancer. The study of circulating tumor cells (CTCs), including their genomic mutations, gene expression profiles, and cell traits, is a primary focus of this research.  Analyses of CTCs using liquid biopsy relies on cell enumeration as an endpoint or CTC enrichment for subsequent analysis.  However, current protocols often employ CTC isolation methods that rely on low-efficiency antibody capture of expressed cell surface biomarkers or cell enrichment methods that can disrupt CTC gene expression or other aspects of their native biology.

In this webinar we will discuss the considerations for CTC isolation and introduce a novel integrated workflow approach that utilizes the Genesis Cell Isolation System using Celselect Slide technology. The Genesis System uses gentle size-based cell selection that preserves CTC native biology. We will describe how this system is designed to facilitate enrichment, enumeration, and identification of various CTC types on-slide and in parallel to result in unbiased size selection and identification of CTCs and other rare cell types. Downstream interrogation of single or enriched CTCs is compatible with a variety of molecular (Droplet Digital PCR, quantitative PCR, next-generation sequencing) and cellular (fluorescence-activated cell sorting, magnetic-activated cell sorting, flow cytometry) applications.

Join this webinar to discover how to achieve a synergistic and unparalleled degree of insight into both cellular heterogeneity of CTCs as well as the growing demand to understand their unique biomolecular signatures.