Bioradiations Staff
The iTaq™ universal SYBR® Green supermix and iTaq™ universal probes supermix are ready-to-use 2x supermixes formulated for sensitive, efficient, and reproducible qPCR data on any instrument.
iTaq universal supermixes let you:
- Obtain reliable and reproducible gene expression analysis results
- Perform challenging simplex or duplex assays for a wide variety of targets (probes supermix)
- Achieve consistent results across real-time instrument platforms under fast or standard conditions
iTaq universal supermixes contain:
- Advanced buffer formulation for optimal dye-based or probe-based qPCR
- Antibody-mediated hot-start iTaq DNA polymerase for rapid 30 sec activation
- A blend of passive reference dyes for universal instrument compatibility
Performance data for the iTaq™ universal SYBR® Green supermix and the iTaq universal probes supermix are presented below.
iTaq™ Universal SYBR® Green Supermix Performance Data
High Reproducibility and Robust Discrimination
Fig. 1. High reproducibility and robust discrimination. Exceptional reproducibility can be achieved with iTaq™ universal SYBR®Green supermix. Efficient discrimination and reliable quantification can be obtained from a 1.33-fold serial dilution of input template. The β-actin gene was amplified from varying amounts of HeLa cDNA (1 ng to 136 pg). From left to right: 1 ng, 753 pg, 565 pg, 425 pg, 320 pg, 240 pg, 181 pg, and 136 pg. Inset is a magnified view showing robust discrimination and reproducible amplification (six replicates for each input amount). RFU, relative fluorescence units.
Reliable Gene Expression Analysis
Fig. 2. Reliable gene expression analysis. iTaq™ universal SYBR® Green supermix provides reliable gene expression data. The β-actin gene was amplified from HeLa cDNA (100 ng to 100 fg) using the CFX96™ real-time PCR detection system. iTaq™ universal SYBR® Green supermix showed over 90% efficiency over six orders of linear dynamic range (R2 = 0.999, slope = –3.512, actin efficiency = 92.6%) RFU, relative fluorescence units.
Sensitive and Efficient Amplification of Genomic DNA
Fig. 3. Extreme sensitivity. iTaq™ universal SYBR® Green supermix allows robust amplification of genomic DNA. The human GAPDH gene was amplified from gDNA (50 ng to 5 g) using the CFX96 real-time PCR detection system. iTaq™ universal SYBR® Green supermix showed 108.1% GAPDH efficiency over several orders of linear dynamic range (R2 = 0.993, slope = –3.143). RFU, relative fluorescence units.
iTaq Universal Probes Supermix Performance Data
Efficient Gene Expression Analysis
Fig. 1. Efficient gene expression analysis. iTaq universal probes supermix is excellent for gene expression analysis. The human β-actin gene was amplified using FAM-labeled probes from HeLa cDNA (100 ng to 100 fg) using the CFX96 real-time PCR detection system. iTaq universal probes supermix showed 94.3% actin efficiency over 6 orders of linear dynamic range (R2 = 0.999, slope = –3.467). RFU, relative fluorescence units.
Reproducible Quantification
Fig. 2. Reproducible quantification. Exceptional reproducibility can be achieved with iTaq universal probes supermix. Efficient discrimination and reliable quantification can be obtained from a 1.33-fold serial dilution of input template. The β-actin gene was amplified from varying amounts of HeLa cDNA (1 ng to 136 pg). From left to right: 1 ng, 753 pg, 565 pg, 425 pg, 320 pg, 240 pg, 181 pg, and 136 pg. Inset is a magnified view showing robust discrimination and reproducible amplification (six replicates for each input amount). RFU, relative fluorescence units.
Extreme Sensitivity
Fig. 3. Extreme sensitivity — efficient amplification of low-abundance target. iTaq universal probes supermix allows efficient amplification and accurate detection of low-abundance targets. The IL-1β gene was amplified using FAM-labeled probes from HeLa cDNA (100, 10, 1, and 0.1 ng) using the CFX96 real-time PCR detection system. iTaq univesal probes supermix showed sensitive detection IL-1β gene even with very low cDNA inputs. RFU, relative fluorescence units.
Duplex qPCR Under Challenging Conditions
Fig. 4. Duplex qPCR under challenging conditions. Gene expression analysis of two targets using iTaq universal probes supermix on the CFX96 real-time PCR detection system. cDNA from human liver (100 ng) was used in each 20 µl reaction for the amplification of tubulin (HEX-labeled probe 
) and IL-2 (FAM–labeled probe ) both in simplex (A) and duplex (B) reactions. There are no significant differences in quantification cycles (Cqs) in the detection of a low-expressing gene (IL-2) in a duplex or singleplex reaction in the presence of a high-expressing gene (GAPDH). RFU, relative fluorescence units.
Ordering Information
| Catalog# | Description |
| 172-5120 | iTaq™ Universal SYBR® Green Supermix, 2 ml (2 x 1 ml vials), 200 x 20 µl reactions |
| 172-5121 | iTaq™ Universal SYBR® Green Supermix, 5 ml (5 x 1 ml vials), 500 x 20 µl reactions |
| 172-5122 | iTaq™ Universal SYBR® Green Supermix, 10 ml (10 x 1 ml vials), 1000 x 20 µl reactions |
| 172-5124 | iTaq™ Universal SYBR® Green Supermix, 25 ml (5 x 5 ml vials), 2500 x 20 µl reactions |
| 172-5125 | iTaq™ Universal SYBR® Green Supermix, 50 ml (10 x 5 ml vials), 5000 x 20 µl reactions |
| 172-5130 | iTaq Universal Probes Supermix, 2 ml (2 x 1 ml vials), 200 x 20 µl reactions |
| 172-5131 | iTaq Universal Probes Supermix, 5 ml (5 x 1 ml vials), 500 x 20 µl reactions |
| 172-5132 | iTaq Universal Probes Supermix, 10 ml (10 x 1 ml vials), 1000 x 20 µl reactions |
| 172-5134 | iTaq Universal Probes Supermix, 25 ml (5 x 5 ml vials), 2500 x 20 µl reactions |
| 172-5135 | iTaq Universal Probes Supermix, 10 ml (10 x 5 ml vials), 5000 x 20 µl reactions |
SYBR is a trademark of Molecular Probes, Inc. Bio-Rad Laboratories, Inc. is licensed by Molecular Probes, Inc. to sell reagents containing SYBR Green I for use in real-time PCR, for research purposes only.
Bio-Rad’s real-time thermal cyclers are licensed real-time thermal cyclers under Applera’s U.S. Patent Number 6,814,934 B1 for use in research, human in vitro diagnostics, and all other fields except veterinary diagnostics.