Minimize On-Column Aggregates during mAb Purification

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the challenge

The Challenge

Have you seen two-peak elution of target monoclonal antibodies (mAb) after cation exchange (CEX) chromatography? The second elution peak consists of a mixture of monomers and aggregates of your target mAb and it leads to a decrease in your process yield.

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purification tip

Purification Tip

Choosing the optimal resin for your process will minimize on-column aggregates and maximize recovery. Be sure to screen multiple resins during your mAb purification process development.

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our approach

Our Approach

On-column aggregate formation depends on the specific chromatography resin used. Resin structure, surface extenders, pore size and distribution each contribute to on-column aggregate formation. Bio-Rad’s Nuvia HR-S Resin — a strong cation exchanger with a pore size of 50 µm without surface extenders — minimizes on-column aggregates. Nuvia HR-S generates only 10% aggregate formation relative to competing resins under similar experimental conditions.

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See how your resin-of-choice fares in on-column aggregate formation during monoclonal antibody purification.

Select resins by clicking on the buttons on the left.

 

%, on-column aggregates

0 min hold time

1,000 min hold time
44%

Eshmuno S

50%

Capto SP ImpRes

51%

POROS 50 HS

65%

Capto S ImpAct

87%

Fractogel SO3–

10%

Nuvia HR-S

 

Additional Information

Minimize on-column aggregate formation by proper resin screening and subsequent optimization of resin type.

Learn more about the high-resolution Nuvia HR-S Cation Exchange Resin.

Request a sample of Nuvia HR-S Resin.

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