Methods that are highly accurate and precise provide the right answer, consistently. For digital PCR (dPCR), Poisson statistics dictate that the accuracy and precision of results are dependent on the accuracy and precision of sample partitions. This means that to get reliable dPCR results, you need to know your partition volume accurately and your partition volumes need to be consistent.
Only Bio-Rad Droplet Digital PCR (ddPCR) partitions samples using droplet technology. Samples are partitioned into droplets using time-tested systems and in-instrument quality control systems look for and reject rare occurrences of droplets that are either too small or too large. In addition to ddPCR technology having more than 12 years of proven results, the technology has been validated by Australia’s National Measurement Institute (NMI), which has used both optical profilometry and nucleic acid–based methods to validate ddPCR droplet volumes with a precision of 14 picoliters.
Watch this video to learn more.