Bioradiations Staff

Dr. Mark Snyder, a leading expert in the field of protein purification, presents the purification process development for two very different targets, adenovirus and bacterial surface proteins. In both cases, a two-step column process results in a preparation with high yield and activity and very low host cell protein and DNA contamination. Conditions used make the methods suitable for large-scale production. In this webinar you will learn about: Advantages of mixed-mode resins Optimizing purification process development Procedures for purifying adenovirus and bacterial surface proteins  

Explore this protocol for cell and tissue homogenate prep for multiplex assays.

Andy Riddell, Flow Cytometry Core Facility Manager at the Wellcome Trust- MRC Stem Cell Institutes, is expanding his core facility by allowing his researchers to conduct sorting on their own. In doing so, he is now free to explore and answer the challenging flow cytometry questions that require more of his expert time. Find out how the S3e Cell Sorter has allowed Andy that freedom and how easy sorting can be for researchers.

See what new articles and videos are in the April edition of Bioradiations.

Dr. John Bruning (University of Adelaide) and Dr. Jiansheng Wu (Genentech), two leading experts in the field of protein purification, share exciting examples of their research and recent chromatography tools they have used to demonstrate robust and improved efficiencies to their discovery and characterization protein purification workflows.

See what new articles and videos are in the March edition of Bioradiations.

Here is a quick protocol for preparing a cell culture supernatant sample for multiplex biomarker analysis.

Traci Lyons describes how PrimePCR Assays ended her laboratory’s struggle to find a qPCR assay that could reliably detect transcription of the cyclooxygenase-2 (Cox-2) gene, which they had identified as potentially prometastatic. Using the PrimePCR Assay for Cox-2, researchers in her lab were finally able to verify cyclooxygenase-2 knockdown, which is a necessary step toward confirming its role in breast cancer metastasis.

See what new articles and videos are in the February edition of Bioradiations.

Choosing the right primary antibody is an arduous task. A lot of standardization needs to happen before we can determine that the antibody is specific and sensitive enough for the purpose of our experiment. The new PrecisonAb™ Antibodies from Bio-Rad go through vigorous testing and validation before they are deemed suitable, thus eliminating the process of trial and error for researchers. The video describes how the PrecisionAb antibodies are developed.