Bioradiations Staff

High-throughput screening (HTS) is a critical component of the entire drug discovery process. Flow cytometry is an attractive alternative to imaging and plate reader assays for HTS in drug discovery, due to its increased sensitivity and multiplexing capabilities. The ZE5 Cell Analyzer eliminates pain points that have hindered the full adoption of flow cytometry for drug discovery.

Process development scientists, faced with an increasing number of proteins or complex biomolecules, continuously search for robust purification methods that can be rapidly scaled for manufacturing. What are the optimal purification conditions for high throughput and yield? Which resin or resin combination best purifies the desired protein? How can an efficient, yet cost-effective process be established? The answer: use a systematic screening approach. Read on to learn more.

The SEQuoia RiboDepletion Kit employs novel technology to deplete ribosomal RNA while preserving rare transcripts and small RNAs, as well as minimizing the risk of degrading or low abundant samples. The SEQuoia RiboDepletion Kit also offers a streamlined workflow and multiplex compatibility, leading to significant time and cost savings compared to traditional methods. Watch this video to learn more.

The lack of reproducibility in research has been a source of frustration for scientists. Learn why antibody validation is critical for ensuring specificity and what validation methods Bio-Rad is implementing. Our scientists validate the performance of antibodies through CRISPR-Cas9 gene editing by knockout (KO), siRNA knockdown (KD), and immunoprecipitation followed by mass spectrometry (IP-MS).

Downstream process purification of proteins requires a resin with optimized bead size for ideal pressure/flow properties and decent dynamic binding capacity (DBC) that provides production efficiencies and good process economics. Nuvia IMAC Resin provides the mechanical strength, pore structures, ligand density, and particle size distribution required for an operational run at 300 cm/hr with a DBC of 40 mg/ml at 2 bar column backpressure. Protein purification can efficiently be scaled up from milligrams in the lab to kilograms in bioprocess manufacturing. The chemical stability of Nuvia IMAC Resins ensure efficient column regeneration, reproducibility between purification runs, extended column lifetime, and product purity. Watch our video to learn how Nuvia IMAC Resins overcome the challenges associated with process purification of proteins using metal affinity chromatography.

Follow along as a new starry-eyed graduate student learns the ins and outs of life as a research scientist, gaining valuable western blotting tips and tricks. Along for the ride are a seasoned postdoctoral fellow and traditional principal investigator.

Purifying proteins that are not highly expressed can be challenging. Watch this video to learn about a case study demonstrating an efficient workflow for two-step purification of a low-expressing recombinant protein using mixed-mode chromatography.

Feeling stressed? Take a break with this qPCR-themed word search.

The Hanging with My Chromies podcast series is focused on the many surprising applications of chromatography and the stories surrounding them. Join us every month to see what you can learn.

The Brilliant Science podcasts will introduce you to some of the amazing science being done around the world. Join us every month to see what you can learn.