Bioradiations Staff
The Bio-Plex Pro human Th17 cytokine panel is a unique blend of magnetic bead–based multiplex immunoassays for the robust and reproducible measurement of 15+1 soluble proteins involved in the T-helper cell type 17 immune response pathway. This pathway is thought to play a key role in such conditions as:
- Rheumatoid arthritis, lupus, psoriasis, multiple sclerosis, and other autoimmune diseases
- Antimicrobial immunity
- Transplant rejection
- Cancer
Serum cytokine levels from individuals with psoriasis (PSO, N = 8), rheumatoid arthritis (RA, N = 14), rheumatoid factors (RF,
N = 10), and systemic lupus erythematosus (SLE, N = 18). Nonmatching normal (NM, N = 11) serum samples were used as the baseline reference. The t-test function in Bio-Plex Data Pro™ software was used to calculate statistical differences. Only P-values with statistical significance are shown.
The human Th17 assays include several useful human biomarkers. Performance data for these assays are provided below.
The lower limit of quantitation (LLOQ), upper limit of quantitation (ULOQ), limit of detection (LOD), and inter-assay precision %CV are mean data determined from three independent multiplex assays in a serum-based matrix. Intra-assay %CV is the mean of eight standard points run in triplicate within one representative assay. LLOQ and ULOQ are defined as the boundary standard curve points that meet precision and accuracy specifications of 10% intra-assay CV and 80–120% recovery. Data were generated using the magnetic workflow with the Bio-Plex Pro II wash station.
* IL-17A/F values are derived from the singleplex assay format.
** Factor × NIBSC value (pg/ml) = Bio-Plex value (pg/ml). Factors are based on average % recovery of Bio-Plex standards (in the linear range) relative to NIBSC standards run on the same assay plate.
The features that enhance the quality and ease of use of these assays are:
- Reagent kit II, with diluents optimized for serum and plasma samples
- Assay quick guide
- Assay protocol optimized for high-precision and broad-assay working ranges
- 2-level quality controls with premixed kits
Ordering Information
| Catalog# | Description |
Human Th17 Cytokine Assays |
|
| Human Th17 Premixed, All-In-One Kit | |
| 171-AA001M |
Bio-Plex Pro Human Th17 Cytokine Panel, 15-Plex, 1 x 96-well, includes premixed coupled magnetic beads, detection antibodies, standards, 2-level controls, detection antibody diluent, standard diluent HB, sample diluent HB, assay buffer, wash buffer, streptavidin-PE, 96-well flat bottom plate, sealing tape, and instructions for detecting:
IL-17A/F not included |
| Human Th17 Singleplex Sets, 1 x 96-well | |
| 171BA001M | IL-1β |
| 171BA002M | lL-4 |
| 171BA003M | lL-6 |
| 171BA004M | IL-10 |
| 171BA005M | IL-17A |
| 171BA006M | IL-17F |
| 171BA007M | IL-21 |
| 171BA008M | IL-22 |
| 171BA009M | IL-23 |
| 171BA010M | IL-25 |
| 171BA011M | IL-31 |
| 171-BA012M | IL-33 |
| 171BA013M | IFN-γ |
| 171BA014M | sCD40L |
| 171BA015M | TNF-α |
| 171BA016M | IL-17A/F |
| Reagent Kits for Use with Human Th17 Assays | |
| 171304055 | Bio-Plex Pro Reagent Kit ll with Filter Plate, 1 x 96-well, includes detection antibody diluent, standard diluent HB, sample diluent HB, assay and wash buffers, streptavidin-PE, filter plate, and sealing tape, for vacuum separation methods |
| 171304055M | Bio-Plex Pro Reagent Kit ll with Flat Bottom Plate, 1 x 96-well, includes detection antibody diluent, standard diluent HB, sample diluent HB, assay and wash buffers, streptavidin-PE, flat bottom plate, and sealing tape, for magnetic separation methods |
| Human Th17 Standards | |
| 171DA0001 | Bio-Plex Pro Human Th17 Cytokine Standard, pkg of 1 vial, lyophilized mixture of 16 standard antigens |
| 171DA0501 | Bio-Plex Pro Human Th17 Cytokine Standard, pkg of 50 lot-matched vials, lyophilized mixture of 16 standard antigens |