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This issue features how the electrophoresis workflow can be expedited using Bio-Rad’s Mini-PROTEAN® TGX™ precast gels (separation in 15 min), Trans-Blot® Turbo™ transfer system (transfer in 3 min), and Gel Doc™ EZ imaging system (imaging with the push of a button), and how this efficiency helps advance research |
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See how human cytokines are measured using Bio-Plex Pro™ cytokine assays and how the Oriole™ fluorescent gel stain can be used in 2-D SDS-PAGE and MALDI-TOF workflows. Delve into behind-the-scene technical details on how the concept of simplification of imaging emerged as the Gel Doc™ EZ imaging system |
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How do you multiplex across Bio-Plex Pro™ human and mouse cytokine panels? Can Profinity eXact fusion-tag system be applied to eukaryotic expression systems? Find out in this issue and learn how Bio-Rad has innovated electrophoresis over the years with its novel solutions, including the recent long shelf-life Mini-PROTEAN® TGX™ gels |
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Find out the recommended conditions for electroporating mammalian cells using Gene Pulser MXcell™ electroporation system and how to successfully purify and cleave the protein affinity tag using Profinity eXact™ fusion-tag system. Also learn how Bio-Plex® phosphoprotein assays were used to study EGFR signaling in cancer xenografts |
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This issue describes the Bio-Rad solutions for multiplex assays (with the Bio-Plex® suspension array system), obtaining full kinetic profiles of molecular interactions without regeneration (with the ProteOn™ XPR protein interaction array system), and for stain-free visualization of gels (with Criterion Stain Free™ gels) |
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This issue explores the possibilities of using high-resolution melt (HRM) analysis in research on breast cancer, genetics of disease-related pathways, and forensics. Also read about rapid assay development and optimization for small molecule discovery using the ProteOn™ XPR36 protein interaction array system |
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Read about the ProteoMiner™ protein enrichment technology and how it facilitated the discovery of diabetes biomarkers, cardiovascular research, and various proteomics applications. Find out how low volumes of DNA can be amplified using the C1000™ thermal cycler |
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This issue features the second-generation multiplex immunoassays and the development and validation of Bio-Plex Pro™ human cytokine and immunoglobulin isotyping assays. Also learn about the preprogrammed protein A and G purification methods in the Profinia™ protein purification system and electroporation conditions for neuroblastoma cell lines |
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Discover how real-time PCR was used as a tool for evaluating RNAi-mediated gene silencing in neuroblastoma cells and get some optimized transfection protocols using the Gene Pulser MXcell™ electroporation system. Also learn now ProteOn™ GLH sensor chip can be used to study interactions between proteins and small molecules |
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See how multiple western blots can be replaced by a single multiplex immunoassay using the Bio-Plex® suspension array system and how Bio-Plex® phosphoprotein assays were used to study signal transduction in cancer cells. Find out about automated electrophoresis using the Experion automated electrophoresis system |
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This issue features the ProteOnTM XPR36 protein interaction array system and how you can obtain kinetic, specificity, and affinity data from 36 biomolecular interactions in one rapid experiment using this system. It also describes how the ProteOnT™ NLC sensor chip can be used for antibody-antigen, DNA-protein, and protein-protein interactions |
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This issue features the Profinia protein purification system for the purification of affinity-tagged proteins. It also gives tips for normalization methods for qPCR and describes the analysis of protein-protein binding using the ProteOn™ XPR36 protein interaction array system |
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This issue describes the determination of kinetic constants using the ProteOn™ XPR36 protein interaction array system and the large-scale removal of albumin and IgG from human serum using the BioLogic DuoFlow™ chromatography system. See also how CHT™ ceramic hydroxyapatite separates various biological molecules |
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This issue features the ProteOn™ XPR36 protein interaction array system, an SPR biosensor for obtaining real-time, label-free kinetic data from protein-protein interaction studies. Also find out how to monitor the expression, purification, and processing of GST-tagged proteins using the Experion automated electrophoresis system |
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Presented here are some general considerations for minimizing run times and maximizing throughput, optimization strategies, and troubleshooting tips for your PCR and real-time PCR experiments |
