Effective Strategies for Endotoxin Removal in Therapeutic Protein Purification

Endotoxins are common pyrogenic impurities in therapeutic protein preparations that make the next steps in the purification process very difficult. They are negatively charged complex aggregates of lipopolysaccharides released in large amounts from the outer membrane of gram-negative bacteria during mechanical cell lysis and death. Their presence in even minute concentrations in therapeutic protein preparations can cause septic shock, tissue injury, and even death.

In research samples, endotoxins can hinder accurate data screening and immunological readouts. Endotoxins are extremely thermally stable and insensitive to pH changes, making their removal especially challenging.

This webcast will present case studies using various resins that have been successfully used for endotoxin removal.

Key topics covered:

• Ensuring low endotoxin levels in any downstream process
• Overcoming purification challenge
• Proven purification workflows that significantly reduce endotoxin levels for a variety of protein therapeutics

About the presenter: Heidi Jones is the market development manager for chromatography, applications and collaborations at Bio-Rad Laboratories. Over the course of 17 years at Amgen Inc. and BioMarin Pharmaceutical Inc. prior to joining Bio-Rad, Heidi specialized in protein purification as a pilot and bench scale technical scientist for a wide variety of proteins, including monoclonal antibodies, bispecifics, Fc-fusions, peptides, and enzymes. Heidi holds a master’s degree in business administration from Saint Mary’s College of California and a bachelor’s degree in biology from the University of California, Los Angeles.