High-throughput screening (HTS) is a critical component of the entire drug discovery process. Flow cytometry is an attractive alternative to imaging and plate reader assays for HTS in drug discovery, due to its increased sensitivity and multiplexing capabilities. The ZE5 Cell Analyzer eliminates pain points that have hindered the full adoption of flow cytometry for drug discovery. Its high-throughput capable sample handling and software, high speed acquisition, and automation readiness allow a variety of screening applications, from simple to complex, faster than ever.