#3 Steps to a Successful Cell Culture Supernatant Prep for Multiplex Immunoassays

The following steps can help with the success of your multiplex assays.

1. Centrifuge at 1,000 x g for 15 min at 4°C.
2. Supplement with serum (5–10%) or BSA (0.5–1%) to reconstitute the standard if using a serum-free culture supernatant.
3. Typically, cell culture supernatant is analyzed undiluted. However, if it is necessary to dilute the sample so that it is in the working range of the standard curve, ensure the sample contains the same amount of serum or BSA as the standard.
4. Aliquot and store at –80°C if not using sample immediately.